Diagnostic tests

Uranotest Inmunoestatus Parvo

Semi-quantitative detection of canine parvo virus antibodies in blood, serum or plasma. A new approach on treating, preventing and diagnosing canine parvovirus. This allows one to determine the immune status with just one drop of blood, and in under 1 minute.

An excellent complement for diagnosing and treatment parvovirus in animals that were diagnosed as positive with the Parvo-Corona Uranotest.

Format: 1 test

Specs
Purpose: Semi-quantitative detection of antibodies against canine parvovirus so as to determine the immune status in preventing and/or treating CPV infections.
Sample: Blood, serum or plasma.
Sensitivity: 98 % versus inhibition of hemagglutination.
Specificity: 100 % versus inhibition of hemagglutination.
Reading time: 10 minutes
Presentation: Individual 1-test boxes

Characteristics

When the parvovirus infection’s evolution is favourable, the antibodies in the blood increase and the virus decreases. Knowing the titer of antibodies against parvovirus allows us to make decisions, and this is highly useful for:

  • Prognosis in dogs affected by parvovirus.
  • Decision on using hyper-immune plasma or interferon (if the level of antibodies is high, we do not need to use it). • Detection of possible vaccination failures.
  • Determination of the immune status to give reinforcement vaccinations.
  • Determination of the ideal moment for vaccination (when the maternal antibodies decrease).
  • Selection of hyper-immune plasma donors.
  • Determination of the immune status in dogs in at-risk situations to carry out reinforcement vaccinations.
  • Substitution of annual vaccination with immune status determination (vaccinating only if the antibody level is low).

Information for the veterinarian

The URANOTEST PARVO IMMUNE STATUS diagnostic kit is based on the immunochromatographic technique and is designed for the qualitative detection of antibodies for Canine Parvovirus in whole blood, serum and plasma.

The test consists of several overlapped membranes. On one of the membranes, there are a test line (T line) and control line (C line). The lines are not visible before applying the sample. After applying the sample in the appropriate sample well, migration begins by capillarity action through the membrane. If the result is negative, one purple colour band appears in the C area. This line, called control line, always appears, as it is a control line indicating that the test has successfully performed. If the test result is positive, in addition to the control line, a second line will form in the test area (Test line).

The intensity of the test line in comparison with the control line will give an approximate idea about the antibody concentration present in the sample.

  1. Test devices individually packaged in aluminium pouch.
  2. Disposable capillary pipettes for sample collection. The mark at the capillary indicates a volume of 5 µl.
  3. Tubes with anticoagulant (EDTA) for sample collection.
  4. Tubes with buffer solution for sample dilution.
  5. Disposable plastic pipettes.
  6. Instructions for use.
  1. For veterinary use only.
  2. Wear disposable gloves when handling the samples. All samples should be treated as potentially infectious. Wash and disinfect hands after handling. Avoid aerosol formation when dispensing the sample.
  3. To obtain good results, it is important to add the correct sample volume.
  4. Open the device just before use.
  5. All reagents must be at room temperature before performing the test.
  6. Do not use the test if the envelope is damaged or broken.
  7. Do not re-use.
  8. Do not use reagents after the expiry date.
  9. The quality of each component of the kit has been individually assessed for each batch. Do not mix components or reagents from kits with different batch numbers.
DOCUMENTATION FOR THE VETERINARY
DOWNLOAD PDFInsert and direction of usage

* This information is available only to veterinarians. Clicking the download link declares that possess appropriate qualifications to access the content..

Frequently Asked Questions

The semi-quantitative detection of antibodies against canine Parvovirus in serum, plasma or blood in dogs.

The Parvo Immune Status Uranotest allows for a new approach in prevention, prognosis and treatment of this controversial disease.

Yes. This is one of the test’s multiple uses. But you must take into account what a vaccination failure means. Many puppies are not able to develop immunity against certain vaccines, which increases the cases of vaccinated and re-vaccinated animals suffering from the disease. But this circumstance is rarely attributable to the quality and efficacy of vaccines, but rather to physiological aspects which are still not well-understood.

No, the Uranotest detects antibodies of any origin, which acts to rate the protection level against parvovirus. For practical purposes, what we are interested in is knowing the immune state, regardless of the origin behind the immunity.

Yes, taking into account that during the first weeks of life, you must detect an appropriate level of antibodies coming form the mother (if she has an optimum immune level).

Puppies frequently go to the clinic that have been imported and/or come from places where appropriate health protocols were not followed. The experience is that many of these puppies, which are presumably vaccinated and re-vaccinated, do not actually have an appropriate level of protection. By performing a test with the Parvo Immune Status Uranotest, we will have a precise score as to the real immunity level, and we can decide to start the vaccination protocol again or trust in the health protocol followed originally.

Knowing the immune status of a dog affected by parvovirosis is an excellent tool for making decisions. As the infection progresses, antibodies increase and antigens decrease. According to the number of antibodies we find at each moment in time, we can rate the severity and favourable or unfavourable evolution of the infection. If the number of antibodies is high, we can predict good possibilities for survival and evaluate the need to use hyper-immune plasmas or interferon.

Reliability using any of them is comparable.

In this case, you must gently tip the tube so that the diluent mixes with the drop adhering to the sides. You must mix gently, shaking the tube with circular movements to achieve a homogeneous mixture.

You should proceed as if it were for any other laboratory procedure.

The whole blood must be kept refrigerated at between 2ºC and 7ºC for no more than 24 hours. It should never be frozen.

If you are not going to be able to use it before 24 hours, separate the plasma by centrifugation or the serum by sedimentation, and you may keep it at between 2ºC and 7ºC for up to 2 weeks. To preserve it for longer, freezing is recommended.

No, because the strip’s colours may degrade 30 minutes after performing the test, which makes it lose interest as a test for specialist purposes.


We recommend freezing the sample and performing the test again when necessary. If this is the case, you can only freeze serum or plasma, never whole blood.

No, as with any other diagnostic procedure, you must wait until it reaches room temperature. If want to speed this process up, hold the tube between your hands for at least 20 minutes. Many veterinarians do not take this circumstance into account, and incorrect temperature is one of the most frequent causes behind false results.

30 minutes after doing the test, degradation and diffusion of the colours on the strip may occur. This does not happen with all tests, but the recommendation is that the test should always be repeated if the reading was not taken before the indicated time, since this may lead to an erroneous interpretation.

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