Diagnostic tests

Uranotest Uriscreen

The quick, reliable, low-cost tool for correct decision-making in urinary tract infections.

Uranotest® Uriscreen™ is a test for detecting bacteria and somatic cells in urine that makes it possible to diagnose urinary tract infections caused by catalase-positive bacteria (the main cause of urinary tract infections) and the presence of somatic cells in just 2 minutes.

In cases of suspected urinary tract infections, Uranotest® Uriscreen™ enables us to decide which samples need to be sent to the laboratory for culture.

Specs
Purpose: Detection of bacteria and somatic cells in urine that makes it possible to diagnose urinary tract infections
Sample: Urine
Reading time: Less than 2 minutes
Execution time: 1 minute
Presentation: Boxes with 10 tests
Registration Nº: 11024 RD

Characteristics

  • High sensitivity even with low bacterial counts as it detects concentrations from just 50,000 CFU/ml.
  • Negative (-) predictive value greater than 95% (percentage of negatives with Uranotest® Uriscreen™ that truly are negative).
  • Result in less than 2 minutes.
  • Economic, which means it can be used in screening for decision-making.
  • No equipment needed.

Information for the veterinarian

 

1 - Transfer the urine to be analyzed to the supplied test tube using the disposable pipette. Add the urine sample, so that the added volume is contained between the minimum and maximum lines printed on the media (approximately 1.5–2 ml). Use a different test tube for each urine sample.


2 - Add 4 drops of 10% hydrogen peroxide (H2O2) solution to each tube of trial. Mix gently, so as not to foam, for five seconds. The
solution will turn blue.


3 - After 1–2 minutes, observe the formation of foam and check the results. If the result is positive, foam will form on the surface of the liquid. If the result is negative, foam will not form.

Catalase action (bacteria) Expected
result with Uranotest® Uriscreen
Visual result Interpretation Recomendation
Catalase +
Staphylococcus pseudintermedius
Corynebacterium diptheriae
Enterobacteriaceae
Escherichia coli
Citrobacter spp
Enterobacter spp
Klebsiella spp
Proteus spp
Salmonella spp
Pseudomonas spp
Positive Foam is generated on the surface until a complete ring is formed. There may be bacteria and/or leukocytes in the urine suggestive of urinary tract infection due to catalase-positive bacteria. Send the sample to the laboratory for culture and antibiogram. Start treatment with a broad spectrum antibiotic effective for urinary tract infections (amoxi + clavulanic acid or cephalosporin). When you have the culture results, change the antibiotic for a more sensitive one or maintain if the current one is sufficiently sensitive.
Catalase –
Streptococcus spp
Enterococcus spp
Negative, although if the sample has more than 10 leucocytes per field as a consequence of the infection, the result may also be positive. Foam does not form on the surface or an incomplete ring of foam forms. Catalase-positive bacteria not present in the urine. There is no need to send the sample to the Laboratory for a culture, as the test has a negative predictive value of 95 %.

 

Screening
n= 165 dogs and cats
SensitivitySpecificity
Uranotest® Uriscreen™89 %71 %
Determination under microscope by expert staff of the presence of abnormal urinary sediment (pyuria, bacteriuria or both)78 %90 %
Detection limit50.000 CFU/ml
10 leucocytes/field

 

False negatives

Catalase-negative bacteria (Streptococcus, Enterococcus)

Bacterial concentrations below the detection limit.

False positives

Bacteria that not does not grow in cultures (Chlamydia, Trichomonas)

Haematuria

Presence of cells shed from the bladder or kidneys in urine

Presence of tumour cells

 

DOCUMENTATION FOR THE VETERINARY
DOWNLOAD PDFInsert UT Uriscreen DOWNLOAD PDFUranotest Uriscreen Brochure

* This information is available only to veterinarians. Clicking the download link declares that possess appropriate qualifications to access the content..

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